Metabolomics in human diseases and in medicinally important plants by NMR spectroscopy.
Metabolomics using NMR spectroscopy is now a well-established technique for obtaining information regarding the metabolic profile of body fluids, tissue extracts and tissues. “Metabolomics/Metabonomics: is a quantitative measurement of time related multi-parametric metabolic responses of multicellular systems to pathophysiology, exogenous or endogenous stimuli or genetic modification”. Its potential of joining genomics, transcriptomics and proteomics, provides extensive information about a living system, which can be further utilized in drug development, diagnostics and health screening. Under pathological conditions some of these genes and proteins either get up-regulated or down-regulated resulting in the derangement in concentrations of various metabolites or altered metabolic pathways result in new molecular entity suitable for diagnostic evaluation. Similarly, plant metabolomics is more complex and NMR spectroscopy is a commonly applied technique in plant metabolomics and has been used to analyze a range of chemically diverse metabolites including catechins, aliphatic compounds, aromatic compounds, organic acids, phenolics, fatty acids, steroids, sugars and bioactive molecules from the plant extracts. Our group is involved in metabolic profiling of body fluids obtained from patients suffering from, parasitic, meningitis, urosepsis and in spinal cord injury (SCI) with follow-up treatment in order to identify significant metabolic confounders correlating the disease state. Moreover, native tissue based HR-MAS NMR metabolomics in oral, gall bladder and breast cancer for rapid differentiation of malignant and non-malignant tissues. In plant metabolomics, our group is involved in identifying bioactive secondary metabolic entities in medicinally and economically important plants.
-
Salivary Diagnostics : Currently emphasizing on salivary metabolomics for establishing early repair biomarkers with regard to oral diseases. Future plans are to establish saliva as an effective biofluid over traditional diagnostic fluids i.e. serum, urine and CSF etc. Saliva serves better than other biofluid of being Non-invasive and effortless way of its collection.
-
-
Tumor and other Disease Metabolomics : Identifying and establishing the distinguished biomarkers which could aid into successful prognostic markers for tumor models.
-
-
Plant Metabolomics :